Segmentation of nerve fibers using multi-level gradient watershed and fuzzy systems

摘要

ObjectiveThis paper presents an algorithm based on multi-level watershed segmentation combined with three fuzzy systems to segment a large number of myelinated nerve fibers in microscope images. The method can estimate various geometrical parameters of myelinated nerve fibers in peripheral nerves. It is expected to be a promising tool for the quantitative assessment of myelinated nerve fibers in related research.